Overlapping peptide library: mainly used for whole protein scanning. The head tail peptide matrix is a common method for screening linear or continuous epitopes. The design of this peptide library is mainly determined by two key parameters: peptide length and offset number. The protein starts from the N-terminal and gradually intercepts to the C-terminal. After the design, there is likely to be an orphan peptide left. We suggest generally transferring one or several amino acids from the N-terminal to maintain the same length.
Truncation peptide library: used to determine the minimum range of epitopes. The establishment of truncated peptide matrix is generally obtained by systematically removing the amino acids on both sides of the original peptide sequence. Truncation peptide library is used to identify the least amino acid sequence related to activity.
Alanine Peptide Scanning Library: used to identify the effects of peptide sites on biological activity. Ala is systematically and sequentially substituted for each amino acid in the peptide sequence. The peptide library was screened by Ala to identify the effects of a specific amino acid on the structure, function and other biological activities of the whole protein.
Random peptide library: this peptide library is designed to replace the selected amino acid residues with 20 natural amino acids simultaneously and randomly by shotgun approach. The alternative peptides in these libraries may enhance the activity of peptides.
Scrambled peptide library: the design of this type of library is based on the internal replacement of the original peptide amino acid sequence. It is the peptide library with the highest degree of mutation. The disturbed peptide is usually used as a negative control to confirm that a specific sequence is the key to protein function or activity. It is also a random screening tool for discovering new targets.
Ordering Information
