At present, 2H, 15N and 13C are still widely used in non radionuclide labeling because of their safe use and low radioactivity. The labeled amino acids can be directly connected to the peptide according to the normal peptide synthesis method. Stable isotope labeled amino acids that can be provided by ammonia linked organisms include:
Ala(13C3, 15N1)、Ala(15N)、 Asp(13C4, 15N1)、 Cs (13C3, 15N1) 、Gly(13C2)、Gly (13C2,15N1)、Gly(15N1)、Gly(13C2)、Glu(13C5, 15N1）、Ile(13C6, 1y5N1)、Ile(15N1)、Leu(13C6,5N1)、Leu(15N1)、Lys(13C6,15N2)、Lys(13C6)、Met(13C5,15N1)、Pro(13C5,15N1)、Ser(13C3,15N1)、Ser(13C3)、Ser(15N1)、Val(13C5,15N1)、Val(15N1)、Tyr(13C9,15N1) ect.
Ordering Information
